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EGFP-supervillin
(Plasmid #13040)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13040 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    supervillin
  • Alt name
    p205
  • Species
    B. taurus (bovine)
  • Insert Size (bp)
    6000
  • GenBank ID
    AF025996
  • Entrez Gene
    SVIL
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Contains cDNA from nucleotides -80 to 5420. Endogenous ClaI converted to KpnI for 5' cloning site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EGFP-supervillin was a gift from Elizabeth Luna (Addgene plasmid # 13040 ; http://n2t.net/addgene:13040 ; RRID:Addgene_13040)
  • For your References section:

    Domain analysis of supervillin, an F-actin bundling plasma membrane protein with functional nuclear localization signals. Wulfkuhle JD, Donina IE, Stark NH, Pope RK, Pestonjamasp KN, Niswonger ML, Luna EJ. J Cell Sci. 1999 Jul . 112 ( Pt 13)():2125-36. PubMed 10362542