28 pCS plcg 1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13059||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesD. rerio (zebrafish)
Insert Size (bp)3938
Entrez Geneplcg1 (a.k.a. fj46f04, wu:fj46f04, wu:fj57a05)
- Cloning method Restriction Enzyme
- 5′ sequencing primer Sp6
- 3′ sequencing primer EBV rev (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The full-length coding sequence of zebrafish plcg1 was amplified by PCR using cDNA from wild-type embryos using the following oligonucleotides: 5'-TTACTAGTGAACAAACAGGGGAAATG-GCT-3' and 5'-TTTCTAGACTGCTCGGTTTACGCTCGGTTAT-3' and TOPO-cloned into pCR2.1 (Invitrogen) to give pCRplcg1CDS. Additional plcg1 cDNA sequence was obtained or confirmed by cycle sequencing of reverse transcriptase PCR (RT
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:28 pCS plcg 1 was a gift from Nathan Lawson (Addgene plasmid # 13059 ; http://n2t.net/addgene:13059 ; RRID:Addgene_13059)
For your References section:phospholipase C gamma-1 is required downstream of vascular endothelial growth factor during arterial development. Lawson ND, Mugford JW, Diamond BA, Weinstein BM. Genes Dev. 2003 Jun 1. 17(11):1346-51. 10.1101/gad.1072203 PubMed 12782653