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362 pCS Cherry DEST
(Plasmid #13075)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13075 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    na
  • Backbone size w/o insert (bp) 5778

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Must be grown in a strain resistant to ccdB.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cherry
  • Insert Size (bp)
    752

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Cherry is from Roger Tsien.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a H30R mutation in mCherry. This mutation does not affect plasmid function and the construct has been validated for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    362 pCS Cherry DEST was a gift from Nathan Lawson (Addgene plasmid # 13075 ; http://n2t.net/addgene:13075 ; RRID:Addgene_13075)
  • For your References section:

    Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311