pAGN33
(Plasmid #131105)

• Purpose: An integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutants
• Depositing Lab: Riccardo Manganelli
• Publication: Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1038/s41598-019-42319-2.

Backbone

• Vector backbone: pTTP1.B
• Backbone manufacturer: Graham Hatfull (Addgene plasmid # 91723)
• Backbone size w/o insert (bp): 5852
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Kanamycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: P-furA102tetO-pip
• Species: Streptomyces pristinaespiralis
• Mutation: the furA102 promoter was mutated with the insertion of 2 tetO sites

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The plasmid has PfurA102tetO-pip sequence; PfurA102 pip gene belongs to pMY718 plasmid ( Forti F. et al. (2009). J Biotechnol. Mar 25;140(3-4):270-7); the promoter was modified through the insertion of two TetR operator site ( Ehrt S.et al. (2005). Nucleic Acids Res., 33, e21).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence is that of pTTP1B with P-furA 102 tetO- pip cloned into the EcoRI site.