pGAN101
(Plasmid
#131123)
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Purpose(Empty Backbone) Plasmid for overexpression of prion domains to induce prions
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 131123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAG426-GAL-ccdB
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Backbone manufacturerSusan Lindquist
- Backbone size (bp) 8781
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Modifications to backbonemKate2 C-terminal tag
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Vector typeYeast Expression
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Selectable markersURA3
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Tag
/ Fusion Protein
- mKate2 (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pAG426-GAL-ccdB-mKate2, plasmid for overexpression of prion domains to induce prions. Perform an LR reaction to yield an expression clone.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGAN101 was a gift from Ahmad Khalil (Addgene plasmid # 131123 ; http://n2t.net/addgene:131123 ; RRID:Addgene_131123) -
For your References section:
A Genetic Tool to Track Protein Aggregates and Control Prion Inheritance. Newby GA, Kiriakov S, Hallacli E, Kayatekin C, Tsvetkov P, Mancuso CP, Bonner JM, Hesse WR, Chakrabortee S, Manogaran AL, Liebman SW, Lindquist S, Khalil AS. Cell. 2017 Nov 2;171(4):966-979.e18. doi: 10.1016/j.cell.2017.09.041. Epub 2017 Oct 19. 10.1016/j.cell.2017.09.041 PubMed 29056345
