|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||131896||Standard format: Plasmid sent in bacteria as agar stab||1||$75 *|
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Backbone manufacturerThermo Fisher Scientific
- Backbone size w/o insert (bp) 2550
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3297
Entrez GeneSLC12A1 (a.k.a. BSC1, NKCC2)
- Cloning method Gateway Cloning
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pDONR221_SLC12A1 was a gift from RESOLUTE Consortium & Giulio Superti-Furga (Addgene plasmid # 131896 ; http://n2t.net/addgene:131896 ; RRID:Addgene_131896)