pc3XB-ZF61
(Plasmid #13195)

Depositing Lab

Daniel Voytas
Publication

Zinc Finger Consortium Modular Assembly Kit v1.0B (unpublished) ( How to cite )
Sequence Information
- Sequences (2)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	13195	Standard format: Plasmid sent in bacteria as agar stab	1	$75	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pc3XB
(Search Vector Database)
Backbone manufacturer
Modified Invitrogen pcDNA3
Backbone size w/o insert (bp) 5400
Vector type
Mammalian Expression
Selectable markers
Neomycin (select with G418)

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin
Growth Temperature
37°C
Growth Strain(s)
XL1 Blue
Copy number
High Copy

Gene/Insert

Gene/Insert name
ZF61
Insert Size (bp)
90

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site XbaI (not destroyed)
3′ cloning site BamHI (not destroyed)
5′ sequencing primer T7

(Common Sequencing Primers)

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
- Sangamo Zinc-Finger Agreement
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

Part of the Zinc Finger Consortium Modular Assembly Kit v1.0. Finger protein sequence: PGEKPYKCPECGKSFSDPGHLVRHQRTH. Target DNA sequence: GGC. This zinc finger is derived from the Barbas module set.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pc3XB-ZF61 was a gift from Daniel Voytas (Addgene plasmid # 13195 ; http://n2t.net/addgene:13195 ; RRID:Addgene_13195)

pc3XB-ZF61 (Plasmid #13195)