MYH11-NanoLuc-2A-tdTomato
(Plasmid
#132569)
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PurposeDonor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele
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Depositing Lab
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Depositing OrganizationMorgridge Institute for Research
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 132569 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 132569-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 132569-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDNR
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHuman MYH11 knockin homology arms
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Alt nameAAT4, FAA4, SMHC, SMMHC
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SpeciesH. sapiens (human)
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Entrez GeneMYH11 (a.k.a. AAT4, FAA4, SMHC, SMMHC, SMMS-1, VSCM2)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 132569-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 132569-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MYH11-NanoLuc-2A-tdTomato was a gift from James Thomson (Addgene plasmid # 132569 ; http://n2t.net/addgene:132569 ; RRID:Addgene_132569) -
For your References section:
A Human Pluripotent Stem Cell-Based Screen for Smooth Muscle Cell Differentiation and Maturation Identifies Inhibitors of Intimal Hyperplasia. Zhang J, McIntosh BE, Wang B, Brown ME, Probasco MD, Webster S, Duffin B, Zhou Y, Guo LW, Burlingham WJ, Kent C, Ferris M, Thomson JA. Stem Cell Reports. 2019 Jun 11;12(6):1269-1281. doi: 10.1016/j.stemcr.2019.04.013. Epub 2019 May 9. 10.1016/j.stemcr.2019.04.013 PubMed 31080110
Map uploaded by the depositor.
