pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
(Plasmid
#126706)
-
Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence
-
Depositing Lab
-
Depositing OrganizationBoston University Medical Campus
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 126706-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 126706-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonePX458
-
Backbone manufacturerMorrissey Laboratory
-
Vector typeCRISPR
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameACTA2_gRNA1-SpCas9-T2A-GF
-
Alt nameACTA2
-
gRNA/shRNA sequenceunknown
-
SpeciesH. sapiens (human)
-
Entrez GeneACTA2 (a.k.a. ACTSA, SMDYS)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 126706-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 126706-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP was a gift from Darrell Kotton (Addgene plasmid # 126706 ; http://n2t.net/addgene:126706 ; RRID:Addgene_126706) -
For your References section:
Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering. Kwong G, Marquez HA, Yang C, Wong JY, Kotton DN. Stem Cell Reports. 2019 Sep 10;13(3):499-514. doi: 10.1016/j.stemcr.2019.07.014. Epub 2019 Aug 15. 10.1016/j.stemcr.2019.07.014 PubMed 31422908
