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Generation of a Purified iPSC-Derived Smooth Muscle-like Population for Cell Sheet Engineering.
Kwong G, Marquez HA, Yang C, Wong JY, Kotton DN
Stem Cell Reports. 2019 Sep 10;13(3):499-514. doi: 10.1016/j.stemcr.2019.07.014. Epub 2019 Aug 15. PubMed Article

Plasmids from Article

ID Plasmid Purpose
126705pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HRdonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence
126706pU6-ACTA2_gRNA1-SpCas9-T2A-GFPto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Antibodies from Article