phNFM
(Plasmid #132597)

• Purpose: Expresses human NFM
• Depositing Lab: Anthony Brown
• Publication: Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 10.1002/cm.21566. Epub 2019 Nov 6.

Backbone

• Vector backbone: pEGFP-C1 with EGFP sequence removed
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3981
• Modifications to backbone: Before subcloning the hNFM cDNA into the vector backbone, we added a Kozak sequence using a PCR strategy.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEFM
• Alt name: neurofilament protein M
• Species: H. sapiens (human)
• Insert Size (bp): 3209
• Entrez Gene: NEFM (a.k.a. NEF3, NF-M, NFM)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • phNFM.gb
• A portion of this plasmid was derived from a plasmid made by: The human NFM cDNA was provided by Virginia Lee.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry