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phNFM
(Plasmid #132597)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 132597 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1 with EGFP sequence removed
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3981
  • Modifications to backbone
    Before subcloning the hNFM cDNA into the vector backbone, we added a Kozak sequence using a PCR strategy.
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NEFM
  • Alt name
    neurofilament protein M
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3209
  • Entrez Gene
    NEFM (a.k.a. NEF3, NF-M, NFM)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The human NFM cDNA was provided by Virginia Lee.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    phNFM was a gift from Anthony Brown (Addgene plasmid # 132597 ; http://n2t.net/addgene:132597 ; RRID:Addgene_132597)
  • For your References section:

    Charcot-Marie-Tooth disease Type 2E/1F mutant neurofilament proteins assemble into neurofilaments. Stone EJ, Uchida A, Brown A. Cytoskeleton (Hoboken). 2019 Jul;76(7-8):423-439. doi: 10.1002/cm.21566. Epub 2019 Nov 6. 10.1002/cm.21566 PubMed 31574566