piHGpd/G170D
(Plasmid #1328)

• Depositing Lab: Susan Lindquist
• Publication: Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(24):12949-56.

Backbone

• Vector backbone: pRS303 (based on pBluescript)
• Backbone size w/o insert (bp): 5000
• Vector type: Yeast Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GPD promoter-hsp82 ts mutant (G170D)
• Alt name: Yeast Heat Shock Protein 82 temperature-sensitive mutant
• Alt name: HSP82
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3800
• Mutation: G170D
• Entrez Gene: HSP82 (a.k.a. YPL240C, HSP90)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1, BamH1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: n/a
• 3′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid maintained in DH5alpha.
Yeast hsp82 ts mutant expressed from the GPD promoter on a His3 marked integrating plasmid.