plk1 WT GFP-myc
(Plasmid
#132963)
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PurposeTo study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage
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Depositing Lab
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Depositing OrganizationMassachusetts Institute of Technology
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 132963 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 132963-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 132963-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepolo-like kinase 1
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SpeciesH. sapiens (human)
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Entrez GenePLK1 (a.k.a. PLK, STPK13)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 132963-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 132963-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
plk1 WT GFP-myc was a gift from Michael Yaffe (Addgene plasmid # 132963 ; http://n2t.net/addgene:132963 ; RRID:Addgene_132963) -
For your References section:
Functional dynamics of Polo-like kinase 1 at the centrosome. Kishi K, van Vugt MA, Okamoto K, Hayashi Y, Yaffe MB. Mol Cell Biol. 2009 Jun;29(11):3134-50. doi: 10.1128/MCB.01663-08. Epub 2009 Mar 23. 10.1128/MCB.01663-08 PubMed 19307309
