pGENE:EFF-1::mCherry::V5
(Plasmid
#133020)
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PurposeEFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells
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Depositing Lab
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Depositing OrganizationTechnion Research and Development Foundation
Located in Israel -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133020 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 133020-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 133020-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGENE
- Backbone size w/o insert (bp) 4498
- Total vector size (bp) 7411
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEFF-1::mCherry::V5
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Alt namepOA81
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SpeciesC. elegans (nematode)
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Insert Size (bp)2913
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Tag
/ Fusion Protein
- V5; mCherry
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site SpeI (destroyed during cloning)
- 5′ sequencing primer pGENE Fw
- 3′ sequencing primer pGENE Rv
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMohler WA, et al. Dev Cell 2002
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 133020-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 133020-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGENE:EFF-1::mCherry::V5 was a gift from Benjamin Podbilewicz (Addgene plasmid # 133020 ; http://n2t.net/addgene:133020 ; RRID:Addgene_133020) -
For your References section:
Structural basis of eukaryotic cell-cell fusion. Perez-Vargas J, Krey T, Valansi C, Avinoam O, Haouz A, Jamin M, Raveh-Barak H, Podbilewicz B, Rey FA. Cell. 2014 Apr 10;157(2):407-419. doi: 10.1016/j.cell.2014.02.020. 10.1016/j.cell.2014.02.020 PubMed 24725407
Map uploaded by the depositor.
