pKT-PDGFRa-IRES-Katushka
(Plasmid #133081)

• Purpose: Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
• Depositing Lab: Maria Castro
• Depositing Organization: University of Michigan
  Located in United States of America
• Publication: Plasmids for sleeping beauty system (unpublished)

Backbone

• Vector backbone: pKT
• Backbone size w/o insert (bp): 4813
• Total vector size (bp): 8083
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PDGFRa
• Species: M. musculus (mouse)
• Insert Size (bp): 3270
• Entrez Gene: Pdgfra (a.k.a. CD140a, Pdgfr-2)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is prone to transposase insertion. We recommend that you screen multiple colonies to ensure that your isolated sample does not contain a transposase which disrupts an important region of the plasmid.