Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pOPS0517
(Plasmid #133158)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133158 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ST18 (S17 delta pir delta hemA)
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    PU module (PsNifH from pOGG043), SC module (sfGFP from pOGG037), and T module (AGTCGTGCACAT and universal primer binding site)
  • Species
    Synthetic
  • Mutation
    Domesticated for Golden Gate cloning

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The only difference is the sequence: AGTCGTGCACAT . Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. This plasmid is not available as an individual bacterial stab, but can be requested as part of the Plasmid-ID Kit (Kit #1000000168 ).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOPS0517 was a gift from Philip Poole (Addgene plasmid # 133158)
  • For your References section:

    Optimizing Rhizobium-legume symbioses by simultaneous measurement of rhizobial competitiveness and N2 fixation in nodules. Mendoza-Suarez MA, Geddes BA, Sanchez-Canizares C, Ramirez-Gonzalez RH, Kirchhelle C, Jorrin B, Poole PS. Proc Natl Acad Sci U S A. 2020 May 5;117(18):9822-9831. doi: 10.1073/pnas.1921225117. Epub 2020 Apr 21. 10.1073/pnas.1921225117 PubMed 32317381