pAAV-rTH-tTA
(Plasmid #133268)

• Purpose: An AAV genome that expresses tTA from the rat TH promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 10.7554/eLife.48983.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 6655
• Total vector size (bp): 7402
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tTA
• Alt name: Tetracycline transactivator
• Insert Size (bp): 747
• Mutation: *(see below)
• Promoter: rTH

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: AflII (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pAAV-rTH-tTA.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Funded by BRAIN initiative
*Note: The tTA in this plasmid was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).