PurposeAn AAV genome that expresses tTA from the rat TH promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||133268||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6655
- Total vector size (bp) 7402
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Alt nameTetracycline transactivator
Insert Size (bp)747
- Promoter rTH
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site AflII (not destroyed)
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Funded by BRAIN initiative
*Note: The tTA in this plasmid was made from a reverse tetracycline transactivator (rtTA) by changing two amino acids (G19E and P56A). These two residue changes have been shown to be sufficient for the reverse phenotype (10.1073/pnas.130192197).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-rTH-tTA was a gift from Viviana Gradinaru (Addgene plasmid # 133268 ; http://n2t.net/addgene:133268 ; RRID:Addgene_133268)
For your References section:Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. Robinson JE, Coughlin GM, Hori AM, Cho JR, Mackey ED, Turan Z, Patriarchi T, Tian L, Gradinaru V. Elife. 2019 Sep 23;8. pii: 48983. doi: 10.7554/eLife.48983. 10.7554/eLife.48983 PubMed 31545171