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(Plasmid #133421)

  • Purpose
    S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
  • Depositing Lab
  • Sequence Information


Item Catalog # Description Quantity Price (USD)
Plasmid 133421 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    ORF for Nip1 encodes aa 4-156 (not complete ORF), ORF for Tif5 (B6) encodes aa 241-405 (not complete ORF)
  • Entrez Gene
    NIP1 (a.k.a. YMR309C)
  • Entrez Gene
    TIF5 (a.k.a. YPR041W, SUI5)
  • Entrez Gene
    SUI1 (a.k.a. YNL244C, MOF2, RFR1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HMS clone ID number: ScCD00061648.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pst39-NST-clone14 was a gift from Gerhard Wagner (Addgene plasmid # 133421 ; ; RRID:Addgene_133421)