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Addgene
Gerhard Wagner Lab: Wagner Lab plasmids
Unpublished

Plasmids from Article

ID Plasmid Purpose
133421pst39-NST-clone14S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
133422 pGAPZa-STE2-1D4Mutant form of yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
133423pGAPZ-STE2-1D4Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
133424pYES2-STE2-1D4Mutant yeast STE2 coding sequence in vector for expression in yeast S. cerevisiae, with Gal1 promoter.
133427pHIS-GB1-BP1 Human BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.
133430pET-24a-STE2Yeast STE2 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
133431pET-24a-CXCR4Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
133433pPICZaa-STE2-cohisYeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133436pPICZa-STE2-1D4Mutant form of yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133437pPICZac-STE2-1D4Mutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.
133439RG-001Heat 3 domain of human elF4G in pET21 backbone.
133440KE-005Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
133441KE-009Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

Antibodies from Article