pGEX-2TKN-bp1
(Plasmid
#133428)
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PurposeHuman BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133428 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-2TKN
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSREBF1
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SpeciesH. sapiens (human)
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Insert Size (bp)150
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MutationSREBP1a amino acids 1-50 fused to GST
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Entrez GeneSREBF1 (a.k.a. HMD, IFAP2, SREBP1, bHLHd1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: HsCD00063793.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2TKN-bp1 was a gift from Gerhard Wagner (Addgene plasmid # 133428 ; http://n2t.net/addgene:133428 ; RRID:Addgene_133428) -
For your References section:
An ARC/Mediator subunit required for SREBP control of cholesterol and lipid homeostasis. Yang F, Vought BW, Satterlee JS, Walker AK, Jim Sun ZY, Watts JL, DeBeaumont R, Saito RM, Hyberts SG, Yang S, Macol C, Iyer L, Tjian R, van den Heuvel S, Hart AC, Wagner G, Naar AM. Nature. 2006 Aug 10;442(7103):700-4. doi: 10.1038/nature04942. Epub 2006 Jun 21. 10.1038/nature04942 PubMed 16799563
Map uploaded by the depositor.
