ActinS14V.CIB.GFP
(Plasmid #133620)

• Purpose: Expresses beta actin S14V - CIBN - GFP protein fusion
• Depositing Lab: Robert Hughes
• Publication: Salem et al J Biol Chem. 2020 Aug 7;295(32):11231-11245. doi: 10.1074/jbc.RA119.012427. Epub 2020 May 18.

Backbone

• Vector backbone: phCMV - GFP
• Backbone manufacturer: Genelantis
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: betaActin - CIB
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Insert Size (bp): 1701
• Promoter: cmv
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: xhoI (not destroyed)
• 3′ cloning site: hindIII (not destroyed)
• 5′ sequencing primer: cmvF

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/857706v2 for bioRxiv preprint.