pAAV-EF1a-DIO-tdTomato-WPRE
(Plasmid
#133786)
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PurposeCre-dependent tdTomato fluorescent reporter for cell labeling
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133786 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAAV-EF1a-double floxed-TdTomato-WPRE
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Backbone manufacturerkarl deisseroth
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Vector typeMammalian Expression, AAV, Cre/Lox
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepAAV-EF1a-DIO-tdTomato-WPRE
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Insert Size (bp)1427
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a T230I mutation in tdTomato. This mutation is not known to affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-DIO-tdTomato-WPRE was a gift from Michael Stryker (Addgene plasmid # 133786 ; http://n2t.net/addgene:133786 ; RRID:Addgene_133786) -
For your References section:
Experience-dependent structural plasticity at pre- and postsynaptic sites of layer 2/3 cells in developing visual cortex. Sun YJ, Espinosa JS, Hoseini MS, Stryker MP. Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21812-21820. doi: 10.1073/pnas.1914661116. Epub 2019 Oct 7. 10.1073/pnas.1914661116 PubMed 31591211
Map uploaded by the depositor.
