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pCT-tRNA
(Plasmid #133813)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 133813 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAYCU268
  • Vector type
    CRISPR
  • Selectable markers
    SAT1 (resistance to Nourseothricin)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
  • gRNA/shRNA sequence
    Unknown
  • Species
    Synthetic

Cloning Information

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

The plasmid contains the following relevant inserts: 1. C. albicans Autonomously Replicating Sequence 2 for episomal propagation in C. tropicalis; 2. CAS9 sequence expressed from a Meyerozyma guilliermondii promoter; 3. Cassette for the expression of the sgRNA from a Ashbya gossypii RNA pol II promoter. The mature sgRNA is released following RNase Z processing of the upstream tRNA and self-cleavage of the dowstream HDV Ribozyme. The guide targeting the gene of interest can be cloned into the SapI/BspQI digested plasmid. The regulatory elements (species of origin of promoters/terminators) are described in the map.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCT-tRNA was a gift from Geraldine Butler (Addgene plasmid # 133813 ; http://n2t.net/addgene:133813 ; RRID:Addgene_133813)
  • For your References section:

    Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species. Lombardi L, Oliveira-Pacheco J, Butler G. mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 10.1128/mSphere.00125-19. 10.1128/mSphere.00125-19 PubMed 30867327