Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #53282)


Item Catalog # Description Quantity Price (USD)
Plasmid 53282 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    New England BioLabs
  • Backbone size w/o insert (bp) 4245
  • Total vector size (bp) 9424
  • Modifications to backbone
    Plasmid pAC-BETA was digested with EcoRI and partially digested with BamHI and a 7.1 kB fragment was gel-purified. Plasmid pACYC184 was digested with EcoRI and BamHI and a 2.4 kB fragment was gel-purified. The two fragments were ligated together to give plasmid pAC-85b.
  • Vector type
    low copy number bacterial cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Use to confirm the function of the products of genes or cDNAs that are thought to encode a phytoene synthase enzyme, or to screen genomic or cDNA libraries for plasmids containing such genes (crtB) or cDNAs (psy). The plasmid pAdPSY1 can be used as a positive control. Grow in liquid culture on a platform shaker at 28 degrees Celsius in darkness for 2-3 days, or on agar plates at room temperature for 3-7 days. A yellow colony color and an accumulation of beta-carotene is indicative of phytoene synthase activity.
  • Copy number
    Low Copy


  • Gene/Insert name
    crtE, crtY, crtI
  • Species
    Erwinia herbicola Eho10
  • Insert Size (bp)
  • GenBank ID
  • Promoter endogenous promoters

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAC-85b was a gift from Francis X Cunningham Jr (Addgene plasmid # 53282 ; ; RRID:Addgene_53282)
  • For your References section:

    A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Cunningham FX Jr, Gantt E. Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. 10.1007/s11120-007-9210-0 PubMed 17634749