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pCAG-CFP-SaCas9-HF (without sgRNA)
(Plasmid #134470)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 134470 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
  • Alt name
  • Mutation
    R245A, N413A, N419A, R654A
  • Promoter CAG
  • Tag / Fusion Protein
    • SV40NLS-3xFLAG (C terminal on insert)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-CFP-SaCas9-HF (without sgRNA) was a gift from Zongli Zheng (Addgene plasmid # 134470 ; ; RRID:Addgene_134470)
  • For your References section:

    Rationally engineered Staphylococcus aureus Cas9 nucleases with high genome-wide specificity. Tan Y, Chu AHY, Bao S, Hoang DA, Kebede FT, Xiong W, Ji M, Shi J, Zheng Z. Proc Natl Acad Sci U S A. 2019 Sep 30. pii: 1906843116. doi: 10.1073/pnas.1906843116. 10.1073/pnas.1906843116 PubMed 31570596