pCAG-Sox2-IRES-Neo
(Plasmid #13462)

• Depositing Lab: Shinya Yamanaka
• Depositing Organization: Kyoto University, Center for iPS Cell Research and Application
  Located in Japan
• Publication: Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708.
• Citations: 1 Article

Backbone

• Vector backbone: pCAG-gw-IRES-Neo
• Backbone size w/o insert (bp): 6300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Sox2
• Species: M. musculus (mouse)
• Insert Size (bp): 950
• Mutation: None
• Entrez Gene: Sox2 (a.k.a. Sox-2, lcc, ysb)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CAG-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag. If this cDNA is used in a non-tagged construct, a functional protein (missing the first 3 amino acids--MYN) is produced as confirmed by co-immunoprecipitation, reporter assay, and other experiments.

DNA (Catalog # 13462-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 13462-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.