pACYCDuet1-ammA
(Plasmid
#134682)
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PurposeExpresses His6-AmmA in E. Coli
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134682 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepACYCDuet1
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Vector typeBacterial Expression
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Selectable markersChlR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameammA
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SpeciesStreptomyces sp. CNR 698
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Insert Size (bp)171
- Promoter T7
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Tag
/ Fusion Protein
- His6 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACYCDuet1-ammA was a gift from Wilfred van der Donk (Addgene plasmid # 134682 ; http://n2t.net/addgene:134682 ; RRID:Addgene_134682) -
For your References section:
Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. 10.1126/science.aau6232 PubMed 31320540
