mSin1 shRNA #1
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13483||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7032
Vector typeMammalian Expression, Lentiviral, RNAi
Growth in Bacteria
Copy numberHigh Copy
Alt nameshRNA mSin1
SpeciesH. sapiens (human)
Insert Size (bp)60
Entrez GeneMAPKAP1 (a.k.a. JC310, MIP1, SIN1, SIN1b, SIN1g)
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LKO.1 5' (Common Sequencing Primers)
Short hairpin construct specific for human mSin1 (mSin1.1#1, TRC shRNA NM_024117.x-526s1c1) from the RNAi Consortium. The target sequence is 5'-GTTGG GACTT TGGTA TTAGA A-3'.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mSin1 shRNA #1 was a gift from David Sabatini (Addgene plasmid # 13483 ; http://n2t.net/addgene:13483 ; RRID:Addgene_13483)
For your References section:mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias MA, Thoreen CC, Jaffe JD, Schroder W, Sculley T, Carr SA, Sabatini DM. Curr Biol. 2006 Aug 15. ():. 10.1016/j.cub.2006.08.001 PubMed 16919458