mCerulean3-Rap1
(Plasmid
#134928)
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PurposeFluorescently tagged GTPase.
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Depositing Lab
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Depositing OrganizationUniversity of North Carolina at Chapel Hill
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 134928-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 134928-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepXFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRap1B, Cerulean
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SpeciesH. sapiens (human)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 134928-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 134928-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCerulean3-Rap1 was a gift from Klaus Hahn (Addgene plasmid # 134928 ; http://n2t.net/addgene:134928 ; RRID:Addgene_134928) -
For your References section:
Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor. O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig E, Chew TL, Adalsteinsson D, Hahn KM. J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 10.1083/jcb.201903019. Epub 2019 Aug 23. 10.1083/jcb.201903019 PubMed 31444239
Map uploaded by the depositor.
