mCerulean3-Rap1
(Plasmid
#134928)
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PurposeFluorescently tagged GTPase.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134928 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepXFP
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4000
- Total vector size (bp) 5300
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mCerulean3-Rap1 was a gift from Klaus Hahn (Addgene plasmid # 134928 ; http://n2t.net/addgene:134928 ; RRID:Addgene_134928) -
For your References section:
Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor. O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig E, Chew TL, Adalsteinsson D, Hahn KM. J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 10.1083/jcb.201903019. Epub 2019 Aug 23. 10.1083/jcb.201903019 PubMed 31444239
Map uploaded by the depositor.
