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Software for lattice light-sheet imaging of FRET biosensors, illustrated with a new Rap1 biosensor.
O'Shaughnessy EC, Stone OJ, LaFosse PK, Azoitei ML, Tsygankov D, Heddleston JM, Legant WR, Wittchen ES, Burridge K, Elston TC, Betzig E, Chew TL, Adalsteinsson D, Hahn KM
J Cell Biol. 2019 Sep 2;218(9):3153-3160. doi: 10.1083/jcb.201903019. Epub 2019 Aug 23.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
134926Rap1A FLARERap1A biosensor
134927Rap1B FLARERap1B biosensor
134928mCerulean3-Rap1Fluorescently tagged GTPase.
134929RalGDS-YPetFluorescently tagged affinity reagent

Antibodies from Article