pAAVTRE3_Clover
(Plasmid #135179)

• Purpose: AAV expression of TRE(TRE3G)-driven, Clover expression for fluorescent labling
• Depositing Lab: Tetsuo Yamamori
• Publication: Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0. doi: 10.1016/j.neuron.2023.04.028.

Backbone

• Vector backbone: pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (addgene plasmid #35509)
• Backbone manufacturer: addgene plasmid #35509
• Backbone size w/o insert (bp): 4041
• Total vector size (bp): 5176
• Modifications to backbone: Promoter and CDS parts were excised from the backbone plasmid by MluI and BamHI. TRE3G promoter and Clover were inserted.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Clover
• Species: Aequorea victoria
• Insert Size (bp): 717
• Mutation: a variant of GFP
• GenBank ID: JX489388.1
• Promoter: TRE3G

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: nd

Gene/Insert 2

• Gene/Insert name: TRE3G
• Species: Synthetic
• Insert Size (bp): 376

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: nd

Resource Information

• Supplemental Documents:
  • AAVTre3_Clover.ape
• A portion of this plasmid was derived from a plasmid made by: Clover was obtained from addgene plasmid #40259. TRE3G promoter was obtained from pTRE3G-ZsGreen1 of Clontech. The use of TRE3G is licensed from TETsystems Inc.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Tet Systems Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This AAV plasmid has been used in Abe et al., 2018 (PMID 30425625) and Lin et al, 2019 (PMID 30720427).