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pAAVTRE3_tdTomato
(Plasmid #135178)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135178 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pAAV-Ef1a-DIO hChR2(E123T/T159C)-EYFP (addgene plasmid #35509)
  • Backbone manufacturer
    addgene plasmid #35509
  • Backbone size w/o insert (bp) 4041
  • Total vector size (bp) 5887
  • Modifications to backbone
    Promoter and CDS parts were excised from the backbone plasmid by MluI and BamHI. TRE3G promoter and tdTomato were inserted.
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    tdTomato
  • Species
    Discosoma sp.
  • Insert Size (bp)
    1428
  • Mutation
    a variant of DsRed
  • GenBank ID
    KJ081243.1
  • Promoter TRE3G

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer nd
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    TRE3G
  • Species
    Synthetic
  • Insert Size (bp)
    376

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site MluI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer nd
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This AAV plasmid has been used in Abe et al., 2018, (PMID 30425625) and Lin et al, 2019, (PMID 30720427).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAVTRE3_tdTomato was a gift from Tetsuo Yamamori (Addgene plasmid # 135178 ; http://n2t.net/addgene:135178 ; RRID:Addgene_135178)