Skip to main content
Addgene

opto-Rac1
(Plasmid #135396)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135396 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5300
  • Total vector size (bp) 8400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    opto-Rac1
  • Species
    H. sapiens (human); Botrytis cinerea
  • Insert Size (bp)
    3100
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    opto-Rac1 was a gift from Brian Chow (Addgene plasmid # 135396 ; http://n2t.net/addgene:135396 ; RRID:Addgene_135396)
  • For your References section:

    Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Berlew EE, Kuznetsov IA, Yamada K, Bugaj LJ, Chow BY. Photochem Photobiol Sci. 2020 Mar 1;19(3):353-361. doi: 10.1039/c9pp00434c. Epub 2020 Feb 12. 10.1039/c9pp00434c PubMed 32048687