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(Plasmid #164472)


Item Catalog # Description Quantity Price (USD)
Plasmid 164472 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5372
  • Total vector size (bp) 8492
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    opto-RhoA-mCherry_pcDNA3.1 was a gift from Brian Chow (Addgene plasmid # 164472 ; ; RRID:Addgene_164472)
  • For your References section:

    Single-Component Optogenetic Tools for Inducible RhoA GTPase Signaling. Berlew EE, Kuznetsov IA, Yamada K, Bugaj LJ, Boerckel JD, Chow BY. Adv Biol. 2021 Sep;5(9):e2100810. doi: 10.1002/adbi.202100810. 10.1002/adbi.202100810 PubMed 34288599