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pcDNA5-H2B_Halo_T2A_EGFP
(Plasmid #135444)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135444 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 135444-D50 50 μg of DNA in Tris buffer $495
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DNA 135444-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    thermofisher
  • Backbone size w/o insert (bp) 5137
  • Total vector size (bp) 7195
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    See Depositor Comments.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H2B-HaloTag7 and EGFP
  • Insert Size (bp)
    2061
  • Promoter CMV/TetO
  • Tag / Fusion Protein
    • H2B (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note this plasmid appears to be unstable during growth in bacteria. We recommend screening 10 smaller colonies after streaking from the original stab. It may be necessary to cut the plasmid with a single-cutting enzyme, gel purify the high molecular weight band, and religate to restore the plasmid.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-H2B_Halo_T2A_EGFP was a gift from Kai Johnsson (Addgene plasmid # 135444 ; http://n2t.net/addgene:135444 ; RRID:Addgene_135444)

  • For your References section:

    Photoactivation of silicon rhodamines via a light-induced protonation. Frei MS, Hoess P, Lampe M, Nijmeijer B, Kueblbeck M, Ellenberg J, Wadepohl H, Ries J, Pitsch S, Reymond L, Johnsson K. Nat Commun. 2019 Oct 8;10(1):4580. doi: 10.1038/s41467-019-12480-3. 10.1038/s41467-019-12480-3 PubMed 31594948
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