pSP64-AGS-mCherry
(Plasmid
#135596)
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PurposeExpression of AGS-mCherry mRNA for microinjection for visualization of AGS protein dynamics in S. purpuratus
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Depositing Lab
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Depositing OrganizationBrown University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 135596-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 135596-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSP64
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Vector typeIn vitro transcription (mRNA synthesis)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAGS-mCherry
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SpeciesS. purpuratus
- Promoter Unknown
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Tag
/ Fusion Protein
- mCherry (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer Unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 135596-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 135596-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP64-AGS-mCherry was a gift from Mamiko Yajima (Addgene plasmid # 135596 ; http://n2t.net/addgene:135596 ; RRID:Addgene_135596) -
For your References section:
Evolutionary modification of AGS protein contributes to formation of micromeres in sea urchins. Poon J, Fries A, Wessel GM, Yajima M. Nat Commun. 2019 Aug 22;10(1):3779. doi: 10.1038/s41467-019-11560-8. 10.1038/s41467-019-11560-8 PubMed 31439829
