pFastBac SpyTag-β2AR-SpyTag
(Plasmid #135620)

• Purpose: Expression of SpyTag-B2AR in insect cell
• Depositing Lab: Hee-Jung Choi
• Publication: Choi et al Science. 2019 Nov 29;366(6469):1150-1156. doi: 10.1126/science.aaw8208.

Backbone

• Vector backbone: pFastBac
• Backbone size w/o insert (bp): 5693
• Total vector size (bp): 6794
• Modifications to backbone: Flag tag on N terminus, eGFP-10xHis-R1D4 tag on C terminus have been added.
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Beta 2 adrenergic receptor
• Alt name: B2AR
• Species: H. sapiens (human)
• Insert Size (bp): 1101
• Mutation: Deleted 1-24 aa, 366-413 aa
• Entrez Gene: ADRB2 (a.k.a. ADRB2R, ADRBR, ARB2, B2AR, BAR, BETA2AR)
• Tags / Fusion Proteins:
  • Spytag (N terminal on insert)
  • Spytag (C terminal on insert)
  • eGFP-10xHis-R1D4 tag (C terminal on backbone)
  • HA-FLAG tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Cla1 (not destroyed)
• 3′ cloning site: Nhe1 (not destroyed)
• 5′ sequencing primer: GGA TTA TTC ATA CCG TCC CA
• 3′ sequencing primer: CGT CGC CGT CCA GCT CGA CCA G

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry