Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1357||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5200
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameYepGAL GSP1
SpeciesS. cerevisiae (budding yeast)
Entrez GeneGSP1 (a.k.a. YLR293C, CNR1, CST17)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer M13R
- 3′ sequencing primer M13F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
pPS293 has the GAL1 promoter (EcoRI/XbaI fragment) in YEp352 (2 micron ori).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPS832 was a gift from Pamela Silver (Addgene plasmid # 1357 ; http://n2t.net/addgene:1357 ; RRID:Addgene_1357)
For your References section:The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Schlenstedt G, Saavedra C, Loeb JD, Cole CN, Silver PA. Proc Natl Acad Sci U S A 1995 Jan 3;92(1):225-9. 10.1073/pnas.92.1.225 PubMed 7816822