integrin alpha9 / alpha5 pcDNA1 neo
(Plasmid #13588)

• Depositing Lab: Dean Sheppard
• Publication: Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25.

Backbone

• Vector backbone: pcDNAI neo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 7000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Tetracycline, 100 & 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Growth instructions: Plasmid contains SupF. Grow in TOP10/P3 cells.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: integrin alpha 9 / alpha 5
• Alt name: ITGA9
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Mutation: Integrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 5
• GenBank ID: NM_002207
• Entrez Gene: ITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

See article for cloning details.