integrin alpha9 / alpha5 pcDNA1 neo
(Plasmid
#13588)
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in the United States of America -
Publication
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Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 13588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 13588-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 13588-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNAI neo
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 7000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Tetracycline, 100 & 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Growth instructionsPlasmid contains SupF. Grow in TOP10/P3 cells.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameintegrin alpha 9 / alpha 5
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Alt nameITGA9
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SpeciesH. sapiens (human)
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Insert Size (bp)3200
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MutationIntegrin alpha 9 chimera containing the cytoplasmic domain of integrin alpha 5
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GenBank IDNM_002207
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Entrez GeneITGA9 (a.k.a. ALPHA-RLC, ITGA4L, RLC)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See article for cloning details.
DNA (Catalog # 13588-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 13588-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
integrin alpha9 / alpha5 pcDNA1 neo was a gift from Dean Sheppard (Addgene plasmid # 13588 ; http://n2t.net/addgene:13588 ; RRID:Addgene_13588) -
For your References section:
The cytoplasmic domain of the integrin alpha9 subunit requires the adaptor protein paxillin to inhibit cell spreading but promotes cell migration in a paxillin-independent manner. Young BA, Taooka Y, Liu S, Askins KJ, Yokosaki Y, Thomas SM, Sheppard D. Mol Biol Cell. 2001 Oct . 12(10):3214-25. 10.1091/mbc.12.10.3214 PubMed 11598204
