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Beta1-GFP in pHcgreen
(Plasmid #69804)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69804 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 69804-D50 50 μg of DNA in Tris buffer $495
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DNA 69804-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHcRed1-N1/1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Modifications to backbone
    after cloning Beta1 into pHcRed the RFp fluorophore sequence was excised usin Age1 and Not1, and the GFP sequence from pEGFPN1 cloned into the pHcRed backbone using the same restriction sites
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Beta1A integrin
  • Alt name
    ITGB1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3700
  • GenBank ID
    NM_002211.3
  • Entrez Gene
    ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Kpn1 (unknown if destroyed)
  • 3′ cloning site Age1 (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40A-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Beta1-GFP in pHcgreen was a gift from Martin Humphries (Addgene plasmid # 69804 ; http://n2t.net/addgene:69804 ; RRID:Addgene_69804)

  • For your References section:

    Quantification of integrin receptor agonism by fluorescence lifetime imaging. Parsons M, Messent AJ, Humphries JD, Deakin NO, Humphries MJ. J Cell Sci. 2008 Feb 1;121(Pt 3):265-71. doi: 10.1242/jcs.018440. 10.1242/jcs.018440 PubMed 18216331
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