Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


pET28a His6Flag2AUF1(p37)
(Plasmid #135939)


Item Catalog # Description Quantity Price (USD)
Plasmid 135939 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5255
  • Total vector size (bp) 6176
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    heterogeneous nuclear ribonucleoprotein D
  • Alt name
    AUF1, HNRNPD p37 isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
    heterogeneous nuclear ribonucleoprotein D
  • Entrez Gene
    HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • Flag2 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a His6Flag2AUF1(p37) was a gift from Yimon Aye (Addgene plasmid # 135939 ; ; RRID:Addgene_135939)
  • For your References section:

    Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1. Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y. FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. 10.1096/fj.201901930R PubMed 31665914