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pET28a His6Flag2AUF1(p42)
(Plasmid #135941)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 135941 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5255
  • Total vector size (bp) 6323
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    heterogeneous nuclear ribonucleoprotein D
  • Alt name
    AUF1 p42
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1068
  • GenBank ID
    NM_031369.2
  • Entrez Gene
    HNRNPD (a.k.a. AUF1, AUF1A, HNRPD, P37, hnRNPD0)
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • Flag2 (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 forward
  • 3′ sequencing primer T7 reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a His6Flag2AUF1(p42) was a gift from Yimon Aye (Addgene plasmid # 135941 ; http://n2t.net/addgene:135941 ; RRID:Addgene_135941)
  • For your References section:

    Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1. Poganik JR, Long MJC, Disare MT, Liu X, Chang SH, Hla T, Aye Y. FASEB J. 2019 Nov 1:fj201901930R. doi: 10.1096/fj.201901930R. 10.1096/fj.201901930R PubMed 31665914