|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1362||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4271
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
ECFP, NUF2 3' UTR, integrating vector with TRP1 marker for C-terminal ECFP fusions
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPS1890 was a gift from Pamela Silver (Addgene plasmid # 1362 ; http://n2t.net/addgene:1362 ; RRID:Addgene_1362)
For your References section:Mapping interactions between nuclear transport factors in living cells reveals pathways through the nuclear pore complex. Damelin M, Silver PA. Mol Cell 2000 Jan;5(1):133-40. 10.1016/S1097-2765(00)80409-8 PubMed 10678175
Map uploaded by the depositor.