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pJW31
(Plasmid #136423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 136423 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 136423-D50 50 μg of DNA in Tris buffer $495
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DNA 136423-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC18-mini-Tn7T-LAC
  • Backbone manufacturer
    Kyoung_Hee Choi, Herbert P. Schweizer
  • Backbone size w/o insert (bp) 5815
  • Total vector size (bp) 11380
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    also has Gentamicin resistance
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Cas5
  • Species
    P. aeruginosa
  • Insert Size (bp)
    654

Gene/Insert 2

  • Gene/Insert name
    Cas8
  • Species
    P. aeruginosa
  • Insert Size (bp)
    1764

Gene/Insert 3

  • Gene/Insert name
    Cas7
  • Species
    P. aeruginosa
  • Insert Size (bp)
    870

Gene/Insert 4

  • Gene/Insert name
    Cas3
  • Species
    P. aeruginosa
  • Insert Size (bp)
    2235

Gene/Insert 5

  • Gene/Insert name
    Cas3
  • Species
    P. aeruginosa
  • Insert Size (bp)
    2235

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJW31 was a gift from Joseph Bondy-Denomy (Addgene plasmid # 136423 ; http://n2t.net/addgene:136423 ; RRID:Addgene_136423)

  • For your References section:

    A compact Cascade-Cas3 system for targeted genome engineering. Csorgo B, Leon LM, Chau-Ly IJ, Vasquez-Rifo A, Berry JD, Mahendra C, Crawford ED, Lewis JD, Bondy-Denomy J. Nat Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 10.1038/s41592-020-00980-w. 10.1038/s41592-020-00980-w PubMed 33077967
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