PurposeMammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||136512||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepEGFP N1
- Backbone size w/o insert (bp) 900
- Total vector size (bp) 4800
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Gene/Insert nameLyn nano nano 3HA
Insert Size (bp)900
- Promoter CMV
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lyn-tdnano-HA was a gift from Bianxiao Cui (Addgene plasmid # 136512 ; http://n2t.net/addgene:136512 ; RRID:Addgene_136512)
For your References section:Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). Hope JM, Liu A, Calvin GJ, Cui B. J Mol Biol. 2020 Jun 12;432(13):3739-3748. doi: 10.1016/j.jmb.2020.04.018. Epub 2020 Apr 23. 10.1016/j.jmb.2020.04.018 PubMed 32335036