pSLIK_Zeo_3xflag_Luciferase
(Plasmid
#136533)
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PurposeLentiviral expression vector for an inducible 3xflag-tagged Luciferase
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 136533 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSLIK-Zeo
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Backbone manufacturerAddgene #25736
- Backbone size w/o insert (bp) 12944
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Vector typeMammalian Expression, Lentiviral ; destinatioin vector for gateway cloning
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Bleocin (Zeocin), 100 & 25 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Growth instructions**Grow plasmid in amp+ 25 ug/ml zeo in low-salt LB at 30degC.**
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLuciferase
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SpeciesFirefly
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Tag
/ Fusion Protein
- 3x FLAG (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLIK_Zeo_3xflag_Luciferase was a gift from Kevin Janes (Addgene plasmid # 136533 ; http://n2t.net/addgene:136533 ; RRID:Addgene_136533) -
For your References section:
Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies. Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins KA, Wang CC, Janes KA. Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200. 10.1126/scisignal.aba4200 PubMed 32291314
