|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1368||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6018
Vector typeYeast Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert nameU1A (1-94)
SpeciesH. sapiens (human)
Entrez GeneRNU1-4 (a.k.a. HSD2, HSD5, HSD6, HSD7, RNU1, RNU1D2, RNU1E2, RNU1F1, RNU1G1, RNU1G2, U1B2, U1D2, U1E2, U1F)
/ Fusion Protein
- GFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer M13R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
CEN plasmid, GAL1 promoter
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pPS2035 was a gift from Pamela Silver (Addgene plasmid # 1368 ; http://n2t.net/addgene:1368 ; RRID:Addgene_1368)
For your References section:Pre-mRNA processing factors are required for nuclear export. Brodsky AS, Silver PA. RNA 2000 Dec;6(12):1737-49. 10.1017/S1355838200001059 PubMed 11142374