pKnockinDonor-humanCTCF-miniAID-mClover3
(Plasmid #136883)

• Purpose: knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF
• Depositing Lab: Chunliang Li
• Publication: Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):6699-6713. doi: 10.1093/nar/gkz462.

Backbone

• Vector backbone: pCR-blunt-TOPO
• Backbone manufacturer: thermo fisher
• Backbone size w/o insert (bp): 3500
• Total vector size (bp): 6000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CTCF-knockin-homology arm-miniAID-mClover3
• Alt name: 5'HA-miniAID-mClover3-3'HA
• Species: H. sapiens (human)
• Insert Size (bp): 2573
• Promoter: no promoter
• Tag / Fusion Protein:
  • miniAID-mClover3 (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: sp6
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The miniAID-mClover3 DNA fragment was amplified from pMK290 vector (Plasmid #72828, Addgene)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The miniAID-mClover3 DNA fragment was amplified from pMK290 vector (Plasmid #72828, Addgene)

Please note: The plasmid contains 13bp insertion immediately downstream of the ORF and a single T to G nucleotide mutation in the 3' flanking sequence compared to the depositor's sequence. These changes are not known to affect plasmid function.