pET-Himar-dCas9
(Plasmid #137079)

• Purpose: Himar-dCas9 gene with C-terminal 6x-His tag for protein purification
• Depositing Lab: Harris Wang
• Publication: Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10.1089/crispr.2019.0030. Epub 2019 Nov 19.

Backbone

• Vector backbone: pET151
• Backbone size w/o insert (bp): 5647
• Total vector size (bp): 10864
• Modifications to backbone: removed N-terminal 6xHis tag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The bacterial strain BL21 DE3 or Rosetta 2 DE3 is preferred for downstream applications.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Himar-dCas9
• Species: Synthetic
• Insert Size (bp): 5217
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GATATAGGCGCCAGCAACCGCACCT
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Supplemental Documents:
  • pET Himar-dCas9_bacteria.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry