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pHsSR beta deltaN Flag
(Plasmid #13731)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13731 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28a-derived
  • Backbone size w/o insert (bp) 5332
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Plasmid grows at 37, expression optimal at 30 degrees or 18 degrees
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Signal recognition particle receptor β subunit
  • Alt name
    SRβ
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    639
  • Mutation
    expresses residues 59-271 of Homo sapiens SRβ with a thrombin protease cleavable N-terminal 6xHis tag snd a C-terminal Flag tag
  • Entrez Gene
    SRPRB (a.k.a. APMCF1)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • FLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHsSR beta deltaN Flag was a gift from Thomas Schwartz (Addgene plasmid # 13731 ; http://n2t.net/addgene:13731 ; RRID:Addgene_13731)
  • For your References section:

    Homodimerization of the G protein SRbeta in the nucleotide-free state involves proline cis/trans isomerization in the switch II region. Schwartz TU, Schmidt D, Brohawn SG, Blobel G. Proc Natl Acad Sci U S A. 2006 May 2. 103(18):6823-8. 10.1073/pnas.0602083103 PubMed 16627619